2-Keto-3-deoxy-octonate, a constituent of cell wall lipopolysaccharide preparations obtained from Escherichiacoli
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چکیده
منابع مشابه
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 2-KETO-3-DEOXY-OCTONATE, A CONSTITUENT OF CELL WALL LIPOPOLYSACCHARIDE PREPARATIONS OBTAINED FROM ESCHERICHIA s*
The purpose of this paper is to pres.ent evidence for the occurrence of a 2-keto-3-deoxy-onic acid (Compound I) as a glycosidically bound constituent in preparations of cell wall lipopolysaccharide (LPS) obtained from Escherichia colt 0111-B4 and from a mutant of this organism, E. coli J-5 (Heath -and Elbein, 1962). Based on results obtained by specific calorimetric techniques, chromatography, ...
متن کاملThe enzymatic synthesis of cytidine monophospho-2-keto-3-deoxy-octonate.
The accompanying paper (Heath and Ghalambor, 1963) presents evidence for the occurrence of 2-keto-3-deoxy-octonate @DO) as a glycosidically bound constituent in preparations of cell wall lipopolysaccharide (LPS) obtained from Escherichia cs 0111-B4. The purpose of this paper is to report the biosynthesis of cytidine monophospho-RDO (CMP-KDO) by an enzyme purified from this organism. Materials a...
متن کاملThe Mechanism of 2-keto-3-deoxy-6-phosphogluconic Aldolase.
In the original study of 2-keto-3-deoxy-6-phosphogluconic aldolase (I), and in subsequent experiments, the net synthesis of 2-keto-3-deoxy-6-phosphogluconate from the cleavage products could not be demonstrated. The present experiments were undertaken to clarify this point and to test the conformity of the reaction catalyzed by 2-keto-3-deoxy-6-phosphogluconic aldolase to the general characteri...
متن کاملBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS THR CHEMICAL SYNTHESIS OF 2-K&TO-3-DEOXY-OCTONATR AND ITS CLEAVAGE BY A SPECIFIC ALDOLASEl
Received April 9, 1963 We have previously reported (Heath and Ghalambor, 1963) the occurrence of 2-keto-3-deoxy-octonate @DO) as a glycosidically-bound constituent of cell wall lipopolysaccharide (LPS) of Rscherichia coli 0111-B& and the enzymatic synthesis of CMP-RDO (Ghalambor and Heath, 1963). This report presents evidence for the chemical synthesis of KDO and its enzymatic cleavage (RDO-ald...
متن کاملIdentification of 2-keto-3-deoxy-d-Gluconate Kinase and 2-keto-3-deoxy-d-Phosphogluconate Aldolase in an Alginate-Assimilating Bacterium, Flavobacterium sp. Strain UMI-01
Recently, we identified an alginate-assimilating gene cluster in the genome of Flavobacterium sp. strain UMI-01, a member of Bacteroidetes. Alginate lyase genes and a 4-deoxy-l-erythro-5-hexoseulose uronic acid (DEH) reductase gene in the cluster have already been characterized; however, 2-keto-3-deoxy-d-gluconate (KDG) kinase and 2-keto-3-deoxy-6-phosphogluconate (KDPG) aldolase genes, i.e., f...
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ژورنال
عنوان ژورنال: Biochemical and Biophysical Research Communications
سال: 1963
ISSN: 0006-291X
DOI: 10.1016/0006-291x(63)90535-7